Cellular and Molecular Reproductive Biology

Mammalian sperm must undergo a series of biochemical modifications in the female reproductive tract collectively called capacitation to undergo acrosomal exocytosis, a process that is essential for fertilization. Our laboratory’s goal is to understand the molecular and cellular mechanisms that govern capacitation and acrosomal exocytosis in mammalian sperm.

Cellular and Molecular Reproductive Biology

  • Principal Invetigator

    PhD, Mariano G. Buffone Currículum Vitae

    Staff researcher (National Scientific and Technical Research Council- CONICET)

  •  

    Clara Isabel Marín Briggiler Ph.D. (Staff researcher CONICET)

    Guillermina Maria Luque Ph.D. (Staff researcher CONICET)

    Ana Romarowski, Ph.D.  (Postdoctoral fellow CONICET)

    Lis del Carmen Puga Molina, Ph.D. student (CONICET)

    Paula A. Balestrini, Ph.D. student (CONICET)

    Martina Jabloñski, Ph.D. student (CONICET)

    Liza Jamaica Schiavi Ehrenhaus (Undergraduate student)

     

Area of Research

The ability of the mammalian sperm to acquire fertilizing capacity during its passage through the female tract in a process called capacitation. During this process, sperm acquire the ability to undergo acrosomal exocytosis to penetrate the zona pellucida (extracellular matrix surrounding the oocyte). During acrosomal exocytosis, the outer acrosomal membrane and the plasma membrane fused at multiple points, causing the release and dispersion of the acrosomal content. Exocytosis is a particular type of regulated secretion, which shares much of the same basic mechanisms that have been described in other secretory cells, such as neurons or cells of the endocrine system. Our laboratory studies the molecular mechanisms that govern the acquisition of the ability to perform acrosomal exocytosis during capacitation in mammalian sperm, an essential step in the process of fertilization.

Three main projects drive our research:

1. We study the participation of small GTPases of the RHO superfamily in the signaling pathway that leads to actin polymerization during mouse sperm capacitation. Actin dynamics play a central role in controlling the process of exocytosis in somatic cells as well as in sperm from several mammalian species. In somatic cells, small GTPases of the RHO superfamily are widely known as master regulators of actin dynamics, however its role in sperm has not been studied yet.

2. Using transgenic mice carrying green fluorescent protein (GFP) in their acrosomes and DsRed in the flagellum (Figure 1), we monitor the process of capacitation and acrosomal exocytosis in vivo, while sperm swan towards the metaphase-II arrested eggs through the female reproductive tract. For many years, it was broadly accepted that sperm undergo acrosomal exocytosis upon interaction with the egg´s zona pellucida (ZP). However, this observation was based only on in vitro studies using solubilized ZP or the more aggressive treatment with calcium ionophore. Recent evidence strongly suggests that ZP may not be the physiological inducer of the acrosome reaction. Using the same model we also study the interplay between calcium and acrosomal exocytosis using a live imaging system strategy to monitor both events simultaneously.

3. Capacitation is a complex process of multiple steps that includes plasma membrane reorganization, ion permeability regulation and cholesterol loss among other things. The transport of ion across the membrane and its association with phosphorylation signaling pathways has not been widely studied in human sperm. In this context we study two aspects of this process:

a) The role of CFTR (Cystic Fibrosis Transmembrane Conductance Regulator), a Cl- and HCO3- transporter and how it affects signal transduction networks associated with capacitation in human sperm. In particular the regulation of two key features of the capacitation process, changes in pH levels and changes in the membrane potential (hyperpolarization).

c) The molecular mechanisms associated with the inhibitory effect of decapacitation factors that are present in the seminal plasma.

 

 

Grants

a) Subsidios de Investigación y desarrollo.

 Actives:

  • RO1TW008662. National Institute of Health (NIH). Fogarty International Center. USA. Title: Role of actin cytoskeleton during Mouse sperm acrosomal exocitosis. Period: 3/2012-3/2016.
  • PICT 2012-1175. Agencia Nacional de Promoción Científica y Tecnológica Argentina. Title: Molecular and cellular events associated with acroxomal exocytosis in mammal sperm. Period: 10/2012-10/2015.
  • PIP 112-2011-010074. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Title: Study of proteins and molecular mechanisms involved in the aquisition of the fertilization capacity in mammal sperm. Period: 2012-2014.

No longer actives

  • PICT 2010-2426. Agencia Nacional de Promoción Científica y Tecnológica Argentina. Title: Molecular mechanisms related to acrosomal exocytosis in mammal sperm. Period: 10/2011-10/2013.
Presentations at Scientific Meetings

1.     Src is a connecting player between PKA activity and hyperpolarization during mouse sperm capacitation. Stiva C; La Spina FA; Arranz SA; Visconti PE; Buffone MG; Krapf D. XVI Jornadas Anuales de la Sociedad Argentina de Biología. Chascomus, 2-4 December 2014.

2.     Acrosomal exocytosis of mouse sperm takes place in the oviduct proximal istmus. La Spina, Romarowski A, Puga Molina L, Vitale AM, Hirohashi N, Buffone MG. XVI Jornadas Anuales de la Sociedad Argentina de Biología. Chascomus, 2-4 December 2014.

3.     Characterization of intracellular calcium increase in response to progesterone in mouse sperm. Romarowski A; Sanchez-Cárdenas C; Darszon A; Buffone MG. XVI Jornadas Anuales de la Sociedad Argentina de Biología. Chascomus, 2-4 December 2014.

4.     Src is a connecting player between PKA activity and hyperpolarization during mouse sperm capacitation. Stiva C; La Spina FA; Arranz SA; Visconti PE; Buffone MG; Krapf D. Congreso de la Sociedad Arg. de Investigación Bioquímica y Biología Molecular. Rosario, Noviembre de 2014.

5.     CFTR channels are essential for the activation of AMPc/PKA signaling associated to capacitation and it participates in pH regulation in human sperm. Puga Molina LC, Romarowski A, La Spina FA, Vitale AM, Buffone MG. LIX Reunión científica de la Sociedad Argentina de Investigación Clínica y la Sociedad Argentina de Inmunología. Mar Del Plata 19-22 November 2014.

6.     Redistribution of EGFP protein in the acrosome as an early indicator of acrosomal exocytosis in murine sperm. La Spina FA, Romarowski A, Puga Molina LC, Vitale AM, Hirohashi N, Buffone MG. LIX Reunión científica de la Sociedad Argentina de Investigación Clínica y la Sociedad Argentina de Inmunología. Mar Del Plata 19-22 November 2014.

7.     The activation of small GTPasas RHOA and RAC1 during sperm capacitation leads to the phosphorilation of LIMK/COFILIN, two proteins essential for actin polymerization and acrosomal exocytosis. Romarowski A, La Spina FA, Battistone MA, Cuasnicu P, Vitale AM, Visconti PE, Krapf D, Buffone MG. LIX Reunión científica de la Sociedad Argentina de Investigación Clínica y la Sociedad Argentina de Inmunología. Mar Del Plata 19-22 November 2014.

8.     Study of pH changes during human sperm capacitation. Torres- Rodríguez P, Buffone MG, Puga Molina L, Darszon A, Treviño CL. Sociedad Mexicana de Bioquimica. 2-8 November 2014. Guadalajara, Mexico.

9.     Activation of the small GTPases RhoA/C and Rac1 during mouse sperm capacitation results in phosphorylation of LIMK/COFILIN and is essential for acrosomal exocytosis. Romarowski A, La Spina FA, Battistone MA, Cuasnicu PS, Visconti PE, Buffone MG. 47th Annual Meeting, Society for the Study of Reproduction. Grand Rapids, MI, USA. 19-23 July 2014

10.  New concepts on acrosomal exocytosis. Molecular and cellular aspects. Buffone M. Jornada satélite de SAIC. 18 -19 December 2013.

11.  VCP (valosin containig protein) is localized to the equatorial segment of the sperm acrosome in mice. La Spina F; Romarowski A; Visconti P; Buffone M. XV Jornadas Anuales de la Sociedad Argentina de Biología. Chascomus, 4-6 December 2013.

12.  LIMK1/2 participates in sperm motility mechanisms through a signalling pathway independent of actin polymerisation. Romarowski A, La Spina FA, Battistone MA, Cuasnicu PS, Buffone MG. XV Jornadas Anuales de la Sociedad Argentina de Biología. . Chascomus, 4-6 December 2013.

13.  VCP (valosin containig protein) is localized to the equatorial segment of the sperm acrosome in mice. La Spina F; Romarowski A; Visconti P; Buffone M. LVIII reunión científica anual de la Sociedad Argentina de Investigación Clínica. Mar del Plata, 20-23 November 2013.

14.  Small GTPasas participate in actin polymerisation during capacitation and acrosomal exocytosis in mouse sperm. Romarowski A; Przepiora E; Battistone M; La Spina F.; Cuasnicu P; Visconti P; Buffone M. LVIII reunión científica anual de la Sociedad Argentina de Investigación Clínica. Mar del Plata, 20-23 November 2013

15.  Basic characterization of a proteic entity that reverts capacitation in human semial plasma. Puga Molina L; Verstraeten S; Buffone M. LVIII reunión científica anual de la Sociedad Argentina de Investigación Clínica. Mar del Plata, 20-23 November 2013

16.  β-adrenérgic activation induces changes in the cytoskeleton of MCF-10 cells. Gargiulo L; Rivero E; Romarowski A; Buffone M; Luthy I; Bruzzone A. LVIII reunión científica anual de la Sociedad Argentina de Investigación Clínica. Mar del Plata, 20-23 November 2013

17.  Role of actin cytoskeleton during mouse sperm acrosomal exocytosis. Buffone MG. Royan International Twin Congress on Reproductive Biomedicine and Stem Cells Biology & Technology. Tehran, Iran. 2-4 September 2013.

18.  Relocalization of EGFP as a marker to detect pre-exocitic stages of mouse sperm acrosomal exocytosis. Buffone MG. Frontiers in Reproduction Symposium. Woodshole, MA. USA. June de 2013.

19.  New Concepts about mouse sperm acrosomal exocytosis. Buffone MG. International Congress of Andrology ICA2013. International Society of Andrology. Melbourne, Australia. 24-27 February 2013.

20.  Biochemical characterization of histone modifications associated to epigenetic changes in germ cells and sperm. La Spina F, Romanato M, Brugo Olmedo, S, De Vincentiis S, Calvo JC, Buffone MG. VI Congreso de la Sociedad Argentina de Andrología y de la Federación Iberoamericana de Sociedades de Andrología (ANDRO). Buenos Aires, Argentina. 18-21 April 2012.

21.  Sperm superoxiyde dismutase as a predictor of human sperm mobility recovery after cryopreservation. Buffone MG, Calamera JC, Brugo-Olmedo S, De Vincentis S, Calamera MM, Storey BT, Doncel GF, Alvarez JA. VI Congreso de la Sociedad Argentina de Andrología y de la Federación Iberoamericana de Sociedades de Andrología (ANDRO). Buenos Aires, Argentina. 18-21 April 2012.

22.  Role of actin cytoskeleton during mouse sperm acrosomal exocytosis. Buffone MG, Romarowski A, La Spina F. The 4th Meeting of Allogeneic Authentication in Animals and Plants. University of Tsukuba, Tsukuba, Japan. 10-12 January 2012.

Collaborations with Mexico

Universidad Nacional Autonoma de Mexico (UNAM), Mexico:

Dr Alberto Darzon

Dra. Claudia Treviño

 

Collaborations with USA

University of Massachusetts Amherst, USA:

Dr. Pablo Visconti: Department of Veterinary and Animal Sciences

 

Collaborations with USA

Department of Electrical and Computer Engineering, School of Biomedical Engineering

Dr. Diego Krapf. Department of Electrical and Computer Engineering, School of Biomedical Engineering, 1301 Campus Delivery, Fort Collins, CO 80523, USA.

Collaboration with Rosario, Argentina

Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR).

Dr. Dario Krapf: Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR). Rosario Santa Fe, Argentina

 

Publications in Spanish

1.     Hormona anti-Mülleriana (AMH) como herramienta diagnóstica en la mujer. Rey R, Bedecarrás P, Brugo Olmedo S, De Vincentiis S, Calamera P, Blanco AM, Grinspon R, Freire A, Buffone MG. Revista SAEGRE; 2013 21(3),12-25.

2.     Serum AMH level can predict the risk of cycle cancellation and the chances of good ovarian response, independently of patient's age or FSH. Calamera, P, Buffone, MG, De Vincentiis, S, Rey RA, Olmedo SB. Jornal Brasileiro de Reproducao Assistida. 2013, 17(2),101-108.

3.     Eventos estructurales y mecanismos moleculares que promueven la exocitosis acrosomal y la motilidad en espermatozoides de mamífero. La Spina F, Romarowski A, Buffone MG. Revista de la Sociedad Argentina de Andrología. Vol: 20, Número 2, Diciembre de 2011.

4.     Mecanismos moleculares relacionados con la exocitosis acrosomal en espermatozoides de mamífero. Romarowski A, La Spina F, Buffone MG. Acta Bioquímica Clínica Latinoamericana 2011; 45 (4): 599-719.

5.     Impacto del estrés oxidativo en la capacidad fecundante de los espermatozoides humanos y su relación con los anticuerpos anti-espermatozoides. Calamera JC, Buffone MG, Doncel GF. Revista de la Sociedad Iberoamericana de Información Científica (SIIC). Octubre de 2004.

6.     Evaluación de la Integridad de la Cromatina en espermatozoides humanos. Buffone MG, Calamera JC. Revista de la Sociedad Argentina de Andrología. Vol: 13, Número 1, Julio de 2004.

7.     Tests de Azul de Anilina y Naranja de Acridina como herramientas para evaluar la cromatina espermática conjuntamente con la morfología estricta en pacientes de ICSI. Buffone MG, Brugo Olmedo S, De Vincentiis S, Calamera JC. Revista de Med. Reproductiva. vol 6, N°2, Diciembre de 2003.

8.     Videomicrografía Computarizada, en el estudio del semen humano, 1° parte. Calamera JC, Santillan CA, Buffone MG.  Revista de Med. Reproductiva. vol 5, N°2 Agosto de 2002.

9.     Videomicrografía Computarizada, en el estudio del semen humano, 2° parte. Calamera JC, Santillan CA, Buffone MG.  Revista de Med. Reproductiva. vol 5, N°3, Diciembre de 2002.

10.  Capacitación y Reacción Acrosómica. Buffone MG, Calamera JC. Informe ALAC, Ciencia y Técnica 2: 7-12, 2001.

Publications (last 5 years)
  1. La Spina FA, Puga Molina L, Romarowski A, Vitale AM, Krapf D, Hirohashi N, Buffone MG. Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct. Dev. Biol. (2016). (in press).
  2. Romarowski A; Sanchez Cardenas C; Ramirez Gomez HV; Puga Molina LC; Hernandez Cruz H; Trevino CL; Darszon A; Buffone MG. A specific transitory increase in intracellular calcium induced by progesterone. Biol Reprod (2016). (in press).
  3. Romarowski A; Luque GM; LA Spina FA; Krapf D; Buffone MG. Role of actin cytoskeleton during mammalian sperm acrosomal exocytosis. Adv Anat Embryol Cell Biol. 2016 (in press).
  4. Stival C; Puga Molina L; Buffone MG; Visconti PE; Krapf D. Sperm capacitation and acrosome reaction in mammalian sperm. Adv Anat Embryol Cell Biol. 2016 (in press).
  5. Romarowski A; Battistone MA; La Spina FA; Puga Molina L;  Luque GM; Vitale AM; Cuasnicu PS; Visconti PE; Krapf D; Buffone MG. PKA-dependent phosphorylation of LIMK1 and Cofilin is essential for mouse sperm acrosomal exocytosis. Dev Biol. 2015 Sep 15;405(2):237-49.
  6. Stival C; La Spina FA; Arranz SA; Visconti PE; Buffone MG; Krapf D. Src is the connecting player between PKA activation and hyperpolarization through SLO3 regulation in mouse sperm. J Biol Chem. 2015 Jul 24;290(30):18855-64.
  7. Hirohashi N, La Spina FA, Romarowski A, Buffone MG. Redistribution of the intra-acrosomal EGFP before acrosomal exocytosis in mouse spermatozoa. Reproduction. 2015 Jun;149(6):657-63.
  8. Buffone MG, Wertheimer EV, Visconti PE, Krapf D. Central role of soluble adenylyl cyclase and cAMP in sperm physiology. Biochim Biophys Acta. 2014 Dec;1842(12PB):2610-2620. Review.
  9. La Spina F, Romanato M, Brugo-Olmedo S, De Vincentiis S, Julianelli V, Rivera RM, Buffone MG. Heterogeneous distribution of histone methylation in mature human sperm. J Assist Reprod Genet. 2014 Jan;31(1):45-9.
  10. Caballero-Campo P, Buffone MG, Benencia F, Conejo-García JR, Rinaudo PF, Gerton GL. A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis. J Cell Physiol. 2014 Jan;229(1):68-78.
  11. Brugo Olmedo S, De Vincentiis S, De Martino E, Bedecarrás P, Blanco AM, Freire A, Buffone MG. Rey R. Prediction of reproductive outcomes according to different serum anti-Müllerian hormone levels in females undergoing intracystoplasmic sperm injection. PLoS One. 2013 Sep 17;8(9):e75685.
  12. De Vincentis S, De Martino E, Buffone MG, Brugo-Olmedo S. The use of metaphase I oocytes matured in vitro is associated with embryo multinucleation. Fertil Steril. 2013 Feb;99(2):414-21.
  13. Buffone MG, Calamera JC, Brugo-Olmedo S, De Vincentis S, Calamera MM, Storey BT, Doncel GF, Alvarez JA. Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa. Fertil Steril. 2012 Feb;97(2):293-8.
  14. Buffone MG, Ijiri T, Cao W, Merdiushev T, Aghajanian HK, Gerton GL. Heads or tails? Structural events and molecular mechanisms that promote mammalian sperm acrosomal exocytosis and motility. Mol Reprod Dev. 2012 Jan;79(1):4-18.
  15. Muro Y, Buffone MG, Okabe M, Gerton GL. Function of the Acrosomal Matrix: Zona Pellucida 3 Receptor (ZP3R/sp56) Is Not Essential for Mouse Fertilization. Biol Reprod 2012 Jan 30;86(1):1-6.
  16. Hirohashi N, Gerton GL, Buffone MG. Video imaging of the sperm acrosome reaction during in vitro fertilization. Commun Integr Biol. 2011 Jul;4(4):471-6.
  17. Bedford-Guaus SJ, McPartlin LA, Xie J, Westmiller SL, Buffone MG, Roberson MS, Molecular Cloning and Characterization of Phospholipase C Zeta in Equine Sperm and Testis Reveals Species-Specific Differences in Expression of Catalytically Active Protein. Biol Reprod, 2011 Jul; 85(1): 78-88.
  18. Sosnik J, Buffone MG, Visconti PE. Analysis of CAPZA3 localization reveals temporally discrete events during the acrosome reaction. J Cell Physiol. 2010 Sep;224(3):575-80.
  19. Muray S, Stein P, Buffone MG, Yamashita S, Schultz RM. Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos. Dev Biol. 2010 May 1; 341(1): 205-12.
  20. Buffone MG, Schindler K, Schultz RM. Over-expression of CDC14B causes mitotic arrest and inhibits zygotic genome activation in mouse preimplantation embryos. Cell Cycle. 2009 Dec 1; 8(23).
  21. Buffone MG, Kim KS, Doak, BJ. Rodriguez-Miranda E, Gerton GL. Functional consequences of Cleavage, dissociation, and exocytotic release of ZP3R/sp56 from the mouse sperm acrosomal matrix. J Cell Sci. 2009 Sep 1;122(Pt 17):3153-60.
  22. Pan J, Eckardt SN. Leu A, Buffone MG, Gerton GL, McLaughlin KJ, Wang PJ. Inactivation of nuclear export factor 2 (Nxf2) impairs spermatogenesis in mice. Dev Biology. 2009 Jun 1;330(1):167-74.
  23. Buffone MG., Rodriguez-Miranda E, Storey BT, Gerton GL. Acrosomal Exocytosis of Mouse Sperm Progresses in a Consistent Direction in Response to Zona Pellucida, J Cellular Physiology. 2009 Sep;220(3):611-20.
  24. Buffone MG, Verstraeten SV, Calamera JC, Doncel GF, High Cholesterol Content and Decreased Membrane Fluidity in Human Spermatozoa are Associated with Protein Tyrosine Phosphorylation and Functional Deficiencies. J Androl. 2009 Sep-Oct;30(5):552-8.
  25. Calamera JC, Buffone MG, Brugo-Olmedo S, De Vincentis S, Calamera MM, Storey BT, Doncel GF, Alvarez JA. Effect of thawing temperature on the motility recovery of cryopreserved human spermatozoa. Fertil Steril. 2010 Feb;93(3):789-94.
  26. Buffone MG, Doncel GF, Calamera JC, Verstraeten SV. Capacitation-associated changes in membrane fluidity in asthenozoospermic human spermatozoa. Int. J. Andrology 2009 Aug;32(4):360-75.
  27. Buffone MG, Foster JA, Gerton GL. Role of the acrosomal matrix in fertilization. Int J Dev Biol. 2008; 52(5-6):511-22.
  28. Rodriguez-Miranda E*, Buffone MG*, Edwards SE, Ord TS, Lin K, Sammel MD, Gerton GL, Moss SB, Williams CJ. Extracellular Adenosine 5'-Triphosphate Alters Motility and Improves the Fertilizing Capability of Mouse Sperm. Biol Reprod, 2008 Jul;79(1):164-71. *Coautores
  29. Buffone MG, Zhuang T, Ord TS, Hui L, Moss SB, Gerton GL. Recombinant mouse sperm ZP3-binding protein (ZP3R/sp56) forms a high order oligomer that binds eggs and inhibits mouse fertilization in vitro. J Biol Chem. 2008 May 2;283(18):12438-45.
  30. Edwards SE, Buffone MG, Knee GR, Rossato M, Bonanni G, Masiero S, Ferasin S, Gerton GL, Moss SB, Williams CJ. Effects of extracellular adenosine 5'-triphosphate on human sperm motility. Reprod Sci. 2007 Oct;14(7):655-66.
  31. Cheng Y, Buffone MG, Kouadio M, Goodheart M, Page DC, Gerton GL, Davidson I, Wang PJ. Abnormal sperm in mice lacking the Taf7l gene. Mol Cell Biol. 2007 Apr;27(7):2582-9.
  32. Buffone MG, Brugo-Olmedo S, Calamera JC, Verstraeten SV, Urrutia F, Grippo L, Corbetta JP, Doncel GF.Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele. Mol Reprod Dev. 2006 Dec;73(12):1591-9.
  33. Buffone MG, Calamera JC, Verstraeten S.V, Doncel GF. Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermatozoa of asthenozoospermic patients. Reproduction. 2005 Jun;129(6):697-705.
  34. Marín-Briggiler CI, Chertihin O, Buffone M, Herr J, Vazquez-Levin MH, Visconti PE. Evidence of the presence of calcium/calmodulin-dependent protein kinase IV in human sperm and its involvement in motility regulation. J Cell Sci. 2005 May 1;118(Pt 9):2013-22.
  35. Buffone MG, Doncel GF, Marin Briggiler CI, Vazquez-Levin MH, Calamera JC. Human sperm subpopulations: relationship between functional quality and protein tyrosine phosphorylation. Hum Reprod. 2004 Jan;19(1):139-46.
  36. Calamera J, Buffone M, Ollero M, Alvarez J, Doncel GF. Superoxide dismutase content and fatty acid composition in subsets of human spermatozoa from normozoospermic, asthenozoospermic, and polyzoospermic semen samples. Mol Reprod Dev. 2003 Dec;66(4):422-30.
  37. Marín Briggiler CI, González Echeverría F, Buffone M, Blaquier J, Calamera JC, Tezón JG, Vázquez Levin MH. Calcium requirements for human sperm function in vitro”. Fertil Steril, Vol. 79 (6), 2003. 1396-403.
  38. Calamera JC, Fernández PJ, Buffone MG, Acosta AA, Doncel GF. Effects of long term in vitro incubation of human spermatozoa: functional parameters and catalase effect. Andrologia 33: 79-86, 2001.
Books
  1. La Spina FA, Stival, C, Krapf D, Buffone MG. Molecular and cellular aspects of mammalian Sperm Acrosomal Exocytosis. En “Animal Models and Human Reproduction”. 2016. Wiley Ed. (en prensa).
  2. Buffone MG, De Vincentis S, Brugo-Olmedo S. “Mecanismos de fertilización en mamíferos” en “El ovocito como paciente”. Primera Edición. Editorial Ascune. 2014. Pág. 61-72.
Awards
  • Best poster presentation for “Characterization of intracellular calcium increase in response to progesterone in mouse sperm”. Romarowski A; Sanchez-Cárdenas C; Darszon A; Buffone MG. XVI Jornadas Anuales de la Sociedad Argentina de Biología. Chascomus, 2-4 December 2014.
  • Best poster presentation for “The activation of small GTPasas RHOA and RAC1 during sperm capacitation leads to the phosphorilation of LIMK/COFILIN, two proteins essential for actin polymerization and acrosomal exocytosis”. Romarowski A, La Spina FA, Battistone MA, Cuasnicu P, Vitale AM, Visconti PE, Krapf D, Buffone MG. LIX Reunión científica de la Sociedad Argentina de Investigación Clínica y la Sociedad Argentina de Inmunología. Mar Del Plata 19-22 November 2014.
  • Best oral presentation for “Valosin containig protein/p97) is localized in the equatorial segment of the mouse acrosome and is released during acrosomal exocytosis”. La Spina F; Romarowski A; Vitale A; Visconti P; Buffone M. XV Jornadas Anuales de la Sociedad Argentina de Biología. Chascomus, 4-6 December 2013.
  • Royan International Award in Reproductive Biology. September 2013.
  • Young Andrologist Award. International Society of Andrology (ISA). February 2013.
  • Young Andrologist Award. Argentine Society of Andrology (SAA) y Federación Iberoamericana de Sociedades de Andrología (ANDRO). April, 2012.
  • Visiting Professor Award. Japanese Society for Promotion of Science (JSPS). October, 2011.
  • Best poster presentation for“Functional Consequences of Cleavage, Disassociation and Exocytotic Release of ZP3R/sp56 From the Mouse Sperm Acrosomal Matrix”. 34th Annual Meeting of the American Society of Andrology. Philadelphia, PA. USA. April 4-7 2009
  • Bayard T. Storey Award for Scientific Excellence (2008). Annual Retreat of the Center for Research and Reproduction in Women’s Health.
  • Best Scientific Presentation for “The expression of CCR6 Receptor in sperm is associated with sperm motility and chemotaxis”. XIII Congress of the Spanish Society of Andrology (2007)
  • Fogarty International Award Postdoctoral Fellowship (2005 - 2008).
  • Frontiers in Reproduction fellowship Award. Marine Biology Lab. Woodshole, MA, USA. May and June 2006.
  • Best Scientific Presentation. "The relationship between functional quality and protein phosphorylation in tyrosine residues in human sperm subpopulations". Argentine Congress of Andrology (2003):
Laboratories
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