Some members of the Buffone team recently participated in the SuperResolution (SuperRes) Congress 2024 in Latin America, held from August 22 to 23 in Rio de Janeiro, Brazil.

Dr. Mariano Buffone and Dr. Ana Romarowski were part of the organizing committee, collaborating with microscopy specialists to carry out this highly significant scientific event.

Dr. Martina Jabloñski gave an oral presentation in the session “Single Molecule Localization Microscopy” titled:
“The Helical Structure of Polymerized Actin in the Mouse Sperm Flagellum Undergoes a Structural Change to Cease Motility at the Time of Sperm-Egg Fusion”.
Authors: Jabloñski M, Luque GM, Gómez-Elías MD, Sanchez-Cardenas C, Xu X, de la Vega-Beltran JL, Corkidi G, Linares A, Abonza Amaro VX, Arenas-Hernandez A, Ramos-Godinez MDP, López-Saavedra A, Krapf D, Darszon A, Guerrero A, Buffone MG.

In her presentation, Martina shared her discoveries on the structural changes in the sperm flagellum during fusion with the oocyte.

Additionally, Lucila R. Gómez-Olivieri presented her results in a poster with the work titled:
“CatSper1 KO Sperm Show Reduced Capacity to Undergo Acrosomal Exocytosis Due to Alterations in the Plasma Membrane Potential”.
Authors: Gómez-Olivieri LR, Jabloñski M, Balestrini PA, Novero AG, Ameijeiras P, Magrotti-Messa L, Regge MV, Krapf D, Luque GM, Buffone MG.

Both Martina and Lucila received support from the Chan Zuckerberg Initiative to attend the congress.

Other works presented at the congress included contributions from various members of the Buffone laboratory, in both oral and poster formats, demonstrating the team’s active participation in this important scientific gathering.

*Poster presentation: “Simultaneous High-Resolution Live Imaging of Cytoplasmic and Intracellular Calcium Store Dynamics in Progesterone-Induced Capacitated Mouse Sperm.” Authors: Sánchez-Cárdenas C, Manikis S, Ruiz K, Jabloñski M, Ameijeiras P, Magrotti L, Visconti PE, Darszon A, Buffone MG, Romarowski A.

*Oral presentation: “Super-Resolution Microscopy to Follow Subcellular Relocalization of PKA During Sperm Capacitation.” Authors: Novero AG, Matamoros Volante A, Gomez Olivieri LR, Stival C, Buffone MG, Krapf D.

*Oral presentation: “Reorganization of the Mouse Sperm Flagellum Membrane During Acrosomal Exocytosis.” Authors: Vazquez-Enciso D, Treviño C, Muñoz C, Jabloñski M, Buffone MG, Darszon A, Guerrero A.

*Oral presentation: “Extension of Resolution in Flow Cytometry Images for Nanometric Study in Cellular Populations.” Authors: Abonza V, Guerrero A, Hernández P, Sanchez J, Buffone MG, Jabloñski M, Darszon A.

The congress, funded by the Chan Zuckerberg Initiative, was an incredibly enriching experience for the team, enabling the acquisition of new knowledge, updates on the latest research, and the establishment of connections with leading researchers in the field of advanced microscopy.