Microscope suitable for quantitative analysis of fixed biological samples or live cell imaging and super-resolution image acquisition.
The confocal module has six lasers and a high-sensitivity detection system with spectral separation that allows the emission of fluorophores to be separated even if their spectra overlap.
The Airyscan detector improves the resolution of the confocal system and can achieve a lateral resolution of 140nm.
It has a Total Internal Reflection (TIRF) illumination system and an EMCCD camera for super-resolution.
It has a cell incubator and an automatic focus drift compensator for long-time recordings. Samples should be mounted on slides or 35 to 60-mm Petri dishes.
Temporary courses, z – stacks can be performed, large areas mosaic, and multiple positions can be programmed, and the acquisition can be automated by combining the different modes. It is also possible to carry out FRAP and FRET experiments.